Anti Amodiaquine
mouse monoclonal antibody
Cat.No. HYB 320-04
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Amodiaquine is an antimalarial drug with effects very similar to those of chloroquine. Amodiaquine has been widely used to treat malaria. Due to reports of fatal adverse drug reactions, discontinuation or modification of its use has been suggested.
Amodiaquine is a hapten of molecular weight 267.3 g/mol.
4-amino-2(diethylaminomethyl)phenol a preparation of a carrier protein
HYB 320-04 is specific for the non-quinoline sidechain of amodiaquine. HYB 320-04 can be used for quantitation of amodiaquine in human serum or plasma with a detection limit of app. 2 ng/ml.
HYB 320-04 is specific for the non-quinoline sidechain of amodiaquine
HYB 320-04 reacts with amodiaquine and is able to distinguish from other antimalarial drugs. Crossreactivity towards desethylchloroquine is app. 1%. Crossreactivity towards desethylamodiaquine has not been tested.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:4000 | |
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Not determined |
1. Rowell V, Rowell FJ, Baker A, Laurie D, Sidki AM (1988) A specific ELISA method for determining chloroquine in urine or dried blood spots. Bull World Health Organ 66:211-217.
2. Shenton FC, Bots M, Menon A, Eggelte TA, de Wit M, Greenwood BM (1988) An ELISA test for detecting chloroquine in urine. Trans R Soc Trop Med Hyg 82:216-220.
3. Escande C, Chevalier P, Verdier F, Bourdon R (1990) Sensitive radioimmunoassay and enzyme-linked immunosorbent assay for the simultaneous determination of chloroquine and its metabolites in biological fluids. J Pharm Sci 79:23-27.
